This book offers a practical approach to the essential immunohematology processes conducted in blood centers. The introductory chapter lays the foundation, discussing a blood center's structure, function, and critical roles in healthcare systems. Written for those who are new to the field, the book delves into critical topics, including a comprehensive examination of the principles of immunohematology, focusing on blood group serology and its significance in transfusion medicine, strategies for identifying and resolving blood group discrepancies, ensuring accurate blood typing and safe transfusions. A detailed overview of Coomb's test, its applications, and interpretation of results is explained for the benefit of the students. Key sections cover various crossmatching methods and provision of transfusion support in cases of autoimmune hemolytic anemia (AIHA). Text emphasizes importance of understanding the role of papaincysteine in blood typing and offers detailed instructions on preparing reconstituted blood for double-volume exchange transfusion. Additionally, the second part of the book addresses recent updates in donor selection criteria and the diverse types of blood components available, modification of blood components, alongside practical considerations during component preparation from different types of blood bags and the storage of blood components. It highlights the significance of apheresis techniques, equipment management, quality control measures, and essential practices for maintaining biosafety and managing biomedical waste in blood centers. The book outlines the steps necessary to apply for a blood center license, providing a thorough understanding of regulatory requirements. By blending theoretical knowledge with practical insights, it will serve as an invaluable resource for those working or studying in the field of immunohematology, blood transfusion and blood components.