Principles of Gene Manipulaon and Genomics, seventh edion, is a comprehensively revised, updated and rewrien to encompass basic and advanced gene manipulaon techniques, genome analysis, genomics, transcriptomics, proteomics, and metabolomics. The basic methodologies are presented in the first part of the book, while more advanced gene-transfer techniques are presented in the second part. The third part moves from the basic gene-manipulaon technologies to genomics, transcriptomics, proteomics, and metabolomics, the major branches of highthroughput. The fourth part of the book discusses how recombinant DNA technology and genomics are being applied in the fields of medicine, agriculture, diagnoscs, forensics, and biotechnology. This Indian Adaptaon of the seventh edion of the book contains updated and extended coverage on Far-Western blong, Electroporaon, PCR, Joining of DNA molecules, Primary nucleode sequence database, Genec manipulaon of animals, RNA interference, Muldimensional liquid chromatography, Protein structures, etc. A number of new topics have been added to make the coverage of content complete as per the Indian curriculum requirement. These include YAC, HAC, Revoluon of NGS technologies, Introducon to molecular phylogenec analysis, Organizaon of the regulatory genome in prokaryotes and eukaryotes, Gene regulatory network, Revoluonary tool for transcriptomics, Epigenomics, Introducon to Network biology, etc. It also includes two new appendices on Safety Guidelines of recombinant DNA technology and Risk assessment of microorganisms.