Under the latest Competency Based Undergraduate Curriculum for the Indian Medical Graduate, the MBBS Phase II students require training different from the traditional and conventional methods to a system-based teaching module. This practical manual, which has been thoroughly revised and updated, aims to assist MBBS students to spend quality time in performing relevant experiments under supervision and use this manual as an easy guide to learn experimental procedures. The content of the book minimizes the burden on the students and gives them the opportunity to have ample time in learning. Under the current curriculum, the student is expected to make relevant observations and record them too. It also contains depiction of important related images and figures which gives the student a comprehensive understanding of the subject and is useful for preparation of viva voce for the professional examinations. This edition also contains related clinical questions which will help the students to integrate their knowledge of microbiology into clinical case-based learning. The practical manual will not only guide the students on relevant topics but the competency will simultaneously be assessed by the teachers on his performance on daily basis.